The Kirkland Lab will open at OMRF in April 2021
Chromatin Regulators in Cancer Lab
In my lab we will study how alterations in certain genes drive cancer. Many people know that a mutation in an important gene can lead to cancer, but another cause can be when a cell turns on a gene that is supposed to be off, or turns off a gene that should be on. Similarly, there are two copies of each gene in our cells, but some cancers have more or fewer copies. Breast cancer, in particular, shows these latter features rather than high mutation rates. My lab will study a particular class of genes called Chromatin Regulators that control which genes get turned on or off in a cell and when genes are turned on, how much they are turned on. Chromatin Regulators are altered in up to 50% of cancer patients making them one of the most altered class of genes.
A second issue in cancer is that some cancers have common alterations in certain genes, whereas other cancers rarely or never have alterations in these same genes. My lab will solve this fundamental challenge in biology of understanding why some genes are altered in some particular cancer but not in others.
Together, these findings will allow us to better predict which therapies will benefit each individual patient, harnessing the power or genetic/chemical synergy, allowing for more a personalized medicine approach.
Topo Inhibitor Sensitivity & Breast Cancer Transcriptional Networks
The accessibility of the genome to regulatory, recombination and repair proteins is controlled in part by the opposition between trithorax and polycomb genes that fine-tune accessibility, histone modifications and gene activity. Breast cancer is largely driven by copy-number variation (CNV) and altered gene dosage. What leads to CNV in breast cancer is largely unknown, but CNV may result from structural and organizational changes in the nucleus as a result of altered chromatin regulation. To determine chromatin regulators that may contribute to this phenomenon I collaborated with the lab of Christina Curtis recently published at Nature Medicine. I specifically served as the bridge between the dry and wet lab portions of this study and performed all wet lab experiments. Our results indicate that CRGs operate within a breast cancer specific co-variance transcriptional network that includes members of the classic polycomb and trithorax genes but broadly extends to over 100 chromatin regulators. To validate the extensive chromatin regulatory network, we used a systems biology approach with the aim of achieving a better understanding of chromatin regulator modulation of anthracycline response in breast cancer patients. Since anthracyclines work in part via inhibition of topoisomerase-II (TOP2) on accessible DNA, I hypothesized that CRGs that mediate DNA accessibility might predict anthracycline response. Using cell line datasets and evaluating the interaction between CRG expression and treatment in predicting survival in a metacohort of early-stage breast cancer patients, I identify CRGs whose expression levels dictate anthracycline benefit across the clinical subgroups. Consistent with my hypothesis, CRGs that promote DNA accessibility,including trithorax complex members, were associated with anthracycline sensitivity when highly expressed, whereas CRGs that reduce accessibility were associated with decreased anthracycline sensitivity. Using molecular biology approaches I validated that expression levels of a CR, KDM4B, modulates TOP2 accessibility to chromatin, elucidating a new pathway of anthracycline resistance. Our novel data-driven methodology identifies a collection of chromatin regulatory genes that form a cancer specific covariate network, parts of which predict response to TOP2 inhibitors likely through alteration of chromatin accessibility. This chromatin regulatory network will inform a robust signature of anthracycline (TOP2i) response that is broadly applicable and provides insight for novel therapeutic targeting and with implications for breast cancer patient stratification and treatment decisions.
Epigenome Editing with FIRE-dCas9
The mSWI/SNF complexes are mutated in 20% of tumors making them one of the most mutated CR complexes in cancer4. In the past, the mechanism of action of ATP-dependent chromatin regulators, such as mSWI/SNF was largely explored using in vitro methods based on measurements of nucleosome mobility. These studies were limited by the fact that these assays were not sensitive to tissue-specific chromatin modifications, topology, long-range interactions, and complex patterns of histone modifications as well as patterns of DNA methylation and human disease mutational backgrounds. To circumvent these problems, I developed the FIRE-Cas9 system that allows one to recruit a specific chromatin regulator and follow the consequences with minute-by-minute kinetics on physiologic chromatin, which I published as a co-first author in Nature Communications. I can examine any locus of biologic or medical importance in virtually any cell type using this technique. Because CR complexes have alterations in many human cancers, it is imperative to understand the mechanism of action of these complexes with unparalleled precision as a basis for therapeutic development. The FIRE-Cas9 system that I developed allows one to do this for the first time.
B.A. in molecular and cell biology, Univ. of California, Berkeley, 2003
Ph.D. in molecular, cell and developmental biology, Univ. of California, Santa Cruz, 2013
Postdoctoral Scholar, Stanford University, 2014 – 2021
Honors & Awards
2008-10 University of California, Santa Cruz Graduate Trainee Award
2012-13 President’s Dissertation Year Fellowship, University of California
2013 American Society for Cell Biology Travel Award (Annual Meeting)
2014-16 Stanford University Tumor Biology Trainee Award
American Association for Cancer Research (AACR) 2015 - present
American Society of Cell Biologists (ASCB) 2015 – 2016
Society for Advancement of Chicanos/Hispanics & Native Americans in Science (SACNAS) 2020
Joining OMRF Scientific Staff in 2021
Seoane JA*, Kirkland JG*, Caswell-Jin JL, Crabtree GR, Curtis C. Chromatin regulators mediate anthracycline sensitivity in breast cancer. Nat Med. 2019 Nov;25(11):1721-1727. Epub 2019 Nov 7. (Co-first authors) PMID: 31700186 PMCID: PMC7220800
Braun SMG*, Kirkland JG*, Chory EJ, Husmann D, Calarco JP, Crabtree GR. Rapid and reversible epigenome editing by endogenous chromatin regulators. Nat Commun. 2017 Sep 15;8(1):560. (*Co-first authors) PMID: 28916764 PMCID: PMC5601922
Kirkland JG*, Peterson MR*, Still CD 2nd*, Brueggeman L*, Dhillon N, Kamakaka RT. Heterochromatin formation via recruitment of DNA repair proteins. Mol Biol Cell. 2015 Apr 1;26(7):1395-410. Epub 2015 Jan 28. (Co-first authors) PMID: 25631822 PMCID: PMC4454184
Kirkland JG, Kamakaka RT. Long-range heterochromatin association is mediated by silencing and double-strand DNA break repair proteins. J Cell Biol. 2013 Jun 10;201(6):809-26. PMID: 23733345 PMCID: PMC3678155. PMCID: PMC3678155
Kirkland JG, Raab JR, Kamakaka RT. TFIIIC bound DNA elements in nuclear organization and insulation. Biochim Biophys Acta. 2013 Mar-Apr;1829(3-4):418-24. doi: 10.1016/j.bbagrm.2012.09.006. Epub 2012 Sep 21. PMID: 23000638 PMCID: PMC3552062
Cell Cycle and Cancer Biology Research Program, MS 48
Oklahoma Medical Research Foundation
825 N.E. 13th Street
Oklahoma City, OK 73104
Phone: (405) 271-_____
Fax: (405) 271-7312