The OMRF Next Generation Sequencing (NGS) facility is a universally accessible resource able to provide investigators with massive amounts of DNA sequence in a relatively short period of time. Our Hiseq 2000 is able to generate 3.2 billion reads for a total of 640 Gigabases in a single 10-day run while our Miseq is able to generate 5 million reads for a total of 1.5 Gigabases in a single 24 hour run.
The facility is capable of processing and analyzing all forms of sequencing projects, including whole genome sequencing, custom targeted resequencing including exome capture, RNA-seq, ChIP-seq, and MethylCap-Seq. Study sample sizes can range from singletons to hundreds or even a thousand samples.
For more information contact Dr. Graham Wiley, Room T2101, at wileyg@omrf.org, or (405) 271-2469
Internal OMRF pricing-
Hiseq:
$2165 – 1 Flowcell Lane.
$325 – Library preparation charge (per library).
Miseq:
$870 – 1 Flowcell, 50 cycles.
$1200 – 1 Flowcell, 300 cycles.
$1250 – 1 Flowcell, 500 cycles.
$325 – Library preparation charge (per library).
Outside OMRF academic institution pricing-
Hiseq:
$2265 – 1 Flowcell Lane.
$325 – Library preparation charge (per library).
Miseq:
$970 – 1 Flowcell, 50 cycles.
$1300 – 1 Flowcell, 300 cycles.
$1350 – 1 Flowcell, 500 cycles.
$325 – Library preparation charge (per library).
Commercial entity pricing-
Please contact us.
PO or account numbers must be provided with sample submission. Quotes are available on request.
For more information contact Dr. Graham Wiley, Room T2101, at wileyg@omrf.org, or (405) 271-2469
Please contact Dr. Wiley prior to sample submission. PO or account numbers must be provided with sample submission. Quotes are available on request.
DNA: 1-5 ug in <120ul of 10mM Tris, pH 8 with cold pack
RNA: 1-10 ug in a wet ethanol pellet on dry ice
All nucleic acids should be sent via overnight shipping.
The OMRF NGS facility does not have the capability of providing NGS level analysis support to its users at this time. The following links may be helpful:
The Burrows-Wheeler Aligner (BWA)
http://bio-bwa.sourceforge.net/
The Genome Analysis Toolkit (GATK)
http://www.broadinstitute.org/gsa/wiki/index.php/The_Genome_Analysis_Toolkit
Samtools
http://samtools.sourceforge.net/samtools.shtml
Picard
http://picard.sourceforge.net/index.shtml
Integrative Genomics Viewer (IGV)
http://www.broadinstitute.org/igv/
Vcftools
http://vcftools.sourceforge.net/index.html
SeqAnswers
http://seqanswers.com/forums/index.php
Upon completion of a run all read data will be converted into fastq format and placed on an accessible FTP site. Information on accessing this site will be emailed to the respective researcher as soon as it is available. The FTP site is only a temporary storage site, however, and all data is subject to deletion without notice after 2 weeks.
