My lab is focused on control of the genome in the brain, and other organs, with aging and disease. While you inherit your genes from your mother and father, how these genes are used is controlled, in part, by something called the epigenome. This epigenome is how your genome is organized and packaged in a cell with some parts being ready for use and other parts stored away. The human genome in every cell is about 6.5 ft long which means it has to be very organized in order to fit. We are characterizing how the brain epigenome changes with aging and the influences of sex, diet, and other environmental factors on how the epigenome changes. Working with other OMRF scientists we also study the retina, ovary, muscle, and other organs to maintain health.
The goal of this research is learning how to maintain a ‘youthful’ epigenome with aging as this could help prevent age-related diseases such as Alzheimer’s, Parkinson’s, and dementias.
The central focus of our research is regulation of the genome through epigenetic mechanisms including how stimuli (e.g., heterochronic parabiosis, exercise, diet, early life adversity) cause persistent epigenome changes that effect cellular function throughout life. We use a combination of transgenic mouse approaches (NuTRAP), next generation sequencing, and bioinformatic methods to understand epigenetic processes within specific CNS cell populations (microglia, astrocytes, neurons). Similarly, we examine accumulation of mutations and deletions to the mitochondrial genome with aging and diabetes; pairing these measurements to assessments of mitochondrial function. Our scientific advances over the past decade in aging research have been: a) identifying that the majority of age-related CNS epigenomic changes are sexually divergent in mice and man; b) disproving the genomic hypomethylation with aging hypothesis; c)determining that, from a molecular neurobiology perspective, cognitive decline is associated with activation of specific pathways (e.g., Nogo signaling) and is not simply a more aged phenotype; d) identifying 100s of epigenomic aging clocks in humans of similar performance to existing clocks; e) developing a mouse models for paired epi/genomic, translatomic, and proteomic analysis of specific cell type. Our long-term goal is to mechanistically alter the epigenome to maintain healthy brain function and prevent the neuroinflammation with aging.
B.A., Wake Forest University (English & Chemistry), Winston-Salem, NC, 1994
Ph.D., Wake Forest University School of Medicine (Pharmacology), Winston-Salem, NC, 2001
Post-Doctoral Fellow, Vollum Institute. Oregon Health Sciences University, Portland, OR
Post-Doctoral Fellow, Yerkes National Primate Research Center, Emory University, Atlanta, GA
Honors & Awards
National Research Council Public Policy Fellow, National Academy of Sciences, Washington, D.C., 1999
Pre-doctoral Training Fellow, NIDA, 1999-2001
Post-doctoral Training Fellow, NIDA, 2001-2004
Best poster award, Proteomic Forum, 2003
NARSAD Young Investigator Award, 2004
Samuel F. Hinkle Penn State Outstanding Young Investigator Award, 2010
President’s Associates Presidential Professor, OUHSC, 2019
Gerontological Society of America, 2012-present
Association for Research in Vision and Ophthalmology, 2006-2016
Society for Neuroscience, 1999-2016
Joined OMRF scientific staff in 2019
Chucair-Elliott AJ, Ocañas SR, Pham K, Van Der Veldt M, Cheyney A, Stanford D, Gurley J, Elliott MH, Freeman WM. Translatomic response of retinal Müller glia to acute and chronic stress. Neurobiol Dis 175:105931, 2022 November, PMID: 36423879
Ocañas SR, Ansere VA, Tooley KB, Hadad N, Chucair-Elliott AJ, Stanford DR, Rice S, Wronowski B, Pham KD, Hoffman JM, Austad SN, Stout MB, Freeman WM. Differential Regulation of Mouse Hippocampal Gene Expression Sex Differences by Chromosomal Content and Gonadal Sex. Mol Neurobiol, 2022 May, PMID: 35589920, PMCID: PMC9119800
Ahn B, Ranjit R, Kneis P, Xu H, Piekarz KM, Freeman WM, Kinter M, Richardson A, Ran Q, Brooks SV, Van Remmen H. Scavenging mitochondrial hydrogen peroxide by peroxiredoxin 3 overexpression attenuates contractile dysfunction and muscle atrophy in a murine model of accelerated sarcopenia. Aging Cell:e13569, 2022 February, PMID: 35199907, PMCID: PMC8920438
Chucair-Elliott AJ, Ocanas SR, Stanford DR, Ansere VA, Buettner KB, Porter H, Eliason NL, Reid J, Sharpe AL, Stout MB, Beckstead MJ, Miller BF, Richardson A, Freeman WM*. Inducible cell-specific mouse models for paired epigenetic and transcriptomic studies of microglia and astroglia. Communications Biology. Nov 19 2020. 3(1):693. PMID: 33214681, PMCID: PMC7678837
Hadad N, Unnikrishnan A, Jackson JA, Masser DR, Otalora L, Stanford DR, Richardson A, Freeman WM. Caloric restriction mitigates age-associated hippocampal differential CG and non-CG methylation. Neurobiology of Aging 2018; 67:53-66. PMID: 29631215, PMCID: PMC5955819
Masser DR, Niran Hadad N, Mangold CA, Unnikrishnan A, Ford MM, Giles CB, Georgescu C, Dozmorov MG, Wren JD, Richardson A, Stanford DR, Freeman WM. Sexually divergent DNA methylation patterns with brain aging. Aging Cell 2017; 16:1342-1352. PMID: 28948711, PMCID: PMC5676057
Mangold CA, Wronowski B, Du M, Masser DR, Hadad N, Bixler GV, Brucklacher RM, Ford MM, Sonntag WE, Freeman WM. Sexually divergent induction of microglial-associated neuroinflammation with hippocampal aging. Journal of Neuroinflammation 2017; 14:141. PMID: 28732515 PMCID: PMC5521082
Hadad N, Masser DR, Logan S, Wronowski B, Mangold CA, Clark N, Otalora L, Unnikrishnan A, Ford MM, Giles CB, Wren JD, Richardson A, Sonntag WE, Stanford DR, Freeman W. Absence of genomic hypomethylation or regulation of cytosine-modifying enzymes with aging in male and female mice. Epigenetics Chromatin. 2016 Jul 13;9:30. eCollection 2016. PMID: 27413395 PMCID: PMC4942942
David Stanford, Ph.D.
Ana Chucair-Elliott, Ph.D.
Associate Staff Scientist
Ashley Cheyney, Ph.D.
Assistant Staff Scientist
Sarah Ocanas, Ph.D.
Assistant Laboratory Manager
Research Technician II
Senior Laboratory Technician
Susan "Suzy" Collins
Project Coordinator II