Nanopore PCR and Plasmid Sequencing

Utilizing Oxford Nanopore Technology sequencing platforms we have the ability to sequence entire plasmids and amplicons (>600 bp) without sequencing primers or PCR. Runs are performed Monday, Wednesday, and Friday. For technical questions and assistance, contact Graham Wiley.

• DNA must be 30-50 ng/ul ≥ 10 ul and quantified using a fluorometric method (i.e. Qubit)
• Files returned will include a .fasta sequence file and an .html summary file containing a complete annotation of each plasmid, along with read length and quality histograms
• Sample Submission Cutoffs:
  Samples for Monday runs must be submitted by 5 pm the previous Friday.
  Samples for Wednesday runs must be submitted by 5 pm the previous Tuesday.
  Samples for Friday runs must be submitted by 5 pm the previous Thursday.
• Sample drop-off points are the refrigerators in rooms E324 and C102.
• Accessing your results: Select the “View My Requests” tab in iLab. The file is under your sample manifest.