2000 Scientific Publications

Arthritis and Immunology Research Program

Criswell, L.A., K.L. Moser, P.M. Gaffney, S. Inda, W.A. Ortmann, D. Lin, J.J. Chen, H. Li, C. Gray-McGuire, B.R. Neas, S.S. Rich, J.B. Harley, T.W. Behrens, and M.F. Seldin. 2000. PARP alleles and SLE: failure to confirm association with disease susceptibility . J. Clin. Invest 105:1501-1502.

D’Cruz, D., G. Keser, M.A. Khamashta, H. Direskeneli, I.N. Targoff, F. Miller, and G.R. Hughes. 2000. Antiendothelial cell antibodies in inflammatory myopathies: distribution among clinical and serologic groups and association with interstitial lung disease . J. Rheumatol. 27:161-164.

del Rincon,I, M.Zeidel, E.Rey, J.B.Harley, J.A.James, M.Fischbach, and I.Sanz. 2000. Delineation of the human systemic lupus erythematosus anti-smith antibody response using phage-display combinatorial libraries . J.Immunol. 165:7011-7016.

Gray-McGuire,C., K.L.Moser, P.M.Gaffney, J.Kelly, H.Yu, J.M.Olson, C.M.Jedrey, K.B.Jacobs, R.P.Kimberly, B.R.Neas, S.S.Rich, T.W.Behrens, and J.B.Harley. 2000. Genome Scan of Human Systemic Lupus Erythematosus by Regression Modeling: Evidence of Linkage and Epistasis at 4p16-15.2. Am.J.Hum.Genet. 67:1460-1469.

Korbet, S.M., E.J. Lewis, M.M. Schwartz, M. Reichlin, J. Evans, and R.D. Rohde. 2000. Factors Predictive of Outcome in Severe Lupus Nephritis. Am. J. Kidney Dis. 35:904-914.

* Kurien, B.T., J. Newland, C. Paczkowski, K.L. Moore, and R.H. Scofield. 2000. Association of neutropenia in systemic lupus erythematosus (SLE) with anti-Ro and binding of an immunologically cross-reactive neutrophil membrane antigen . Clin. Exp. Immunol. 120:209-217.

Panush, R.S., M.L. Levine, and M. Reichlin. 2000. Do I need an ANA? Some thoughts about man’s best friend and the transmissibility of lupus [editorial] . J. Rheumatol. 27:287-291.

* Perera,L., C.Foley, T.A.Darden, D.Stafford, T.Mather, C.T.Esmon, and L.G.Pedersen. 2000. Modeling zymogen protein C . Biophys.J. 79:2925-2943.

Rayno, K. and M. Reichlin. 2000. Evaluation of assays for the detection of autoantibodies to the ribosomal P proteins. Clin. Immunol. 95:99-103.

Reichlin, M. 2000. ANA negative systemic lupus erythematosus sera revisited serologically . Lupus 9:116-119.

Reichlin, M. 2000. Anti-telomere antibodies in systemic lupus erythematosus . Lupus 9:317.

Scofield, R.H., D.M. Racila, T.P. Gordon, B.T. Kurien, and R.D. Sontheimer. 2000. Anti-calreticulin segregates anti-Ro sera in systemic lupus erythematosus: anti-calreticulin is present in sera with anti-Ro alone but not in anti-Ro sera with anti-La or anti-ribonucleoprotein . J. Rheumatol. 27:128-134.

Targoff,I.N. 2000. Update on myositis-specific and myositis-associated autoantibodies . Curr.Opin.Rheumatol. 12:475-481.

Teunissen, S.W., M.J. Kruithof, A.D. Farris, J.B. Harley, W.J. Venrooij, and G.J. Pruijn. 2000. Conserved features of Y RNAs: a comparison of experimentally derived secondary structures. Nucleic Acids Res. 28:610-619.

Weser,S., M.Bachmann, K.H.Seifart, and W.Meissner. 2000. Transcription efficiency of human polymerase III genes in vitro does not depend on the RNP-forming autoantigen La. Nucleic Acids Res. 28:3935-3942.

Cardiovascular Biology Research Program

Alberio, L., O. Safa, K.J. Clemetson, C.T. Esmon, and G.L. Dale. 2000. Surface expression and functional characterization of alpha-granule factor V in human platelets: effects of ionophore A23187, thrombin, collagen, and convulxin. Blood 95:1694-1702.

Allen,R.C., D.C. Dale, and F.B.Taylor. 2000. Blood phagocyte luminescence: gauging systemic immune activation. Methods Enzymol. 305:591-629.

Birdwell, B.G., G.E. Raskob, T.L. Whitsett, S.S. Durica, P.C. Comp, J.N. George, T.L. Tytle, W.L. Owen, and P.A. McKee. 2000. Predictive value of compression ultrasonography for deep vein thrombosis in symptomatic outpatients: clinical implications of the site of vein noncompressibility. Arch. Intern. Med. 160:309-313.

Carson, C.W., P.C. Comp, A.R. Rezaie, N.L. Esmon, and C.T. Esmon. 2000. Antibodies to thrombomodulin are found in patients with lupus anticoagulant and unexplained thrombosis . J. Rheumatol. 27:384-390.

Cooper,J.A., G.J.Miller, K.A.Bauer, J.H.Morrissey, T.W.Meade, D.J.Howarth, S.Barzegar, J.P.Mitchell, and R.D.Rosenberg. 2000. Comparison of novel hemostatic factors and conventional risk factors for prediction of coronary heart disease . Circulation 102:2816-2822.

Epperson, T.K., K.D. Patel, R.P. McEver, and R.D. Cummings. 2000. Noncovalent association of p-selectin glycoprotein ligand-1 and minimal determinants for binding to p-selectin. J. Biol. Chem. 275:7839-7853.

Esmon,C.T. 2000. The Anticoagulant and Anti-inflammatory Roles of the Protein C Anticoagulant Pathway. J.Autoimmun. 15:113-116.

Esmon,C.T. 2000. Does inflammation contribute to thrombotic events? Haemostasis 30 Suppl S2:34-40.

Esmon, C.T. 2000. The endothelial cell protein C receptor . Thromb. Haemost. 83:639-643.

Esmon, C.T. 2000. Introduction: are natural anticoagulants candidates for modulating the inflammatory response to endotoxin? . Blood 95:1113-1116.

Esmon,C. 2000. The protein C pathway . Crit Care Med. 28:S44-S48.

Esmon, C.T. 2000. Regulation of blood coagulation. Biochim. Biophys. Acta 1477:349-360.

Esmon,N.L., O.Safa, M.D.Smirnov, and C.T.Esmon. 2000. Antiphospholipid Antibodies and the Protein C Pathway. J.Autoimmun. 15:221-225.

Forlow, S.B., R.P. McEver, and M.U. Nollert. 2000. Leukocyte-leukocyte interactions mediated by platelet microparticles under flow . Blood 95:1317-1323.

Gu, J.M., K. Fukudome, and C.T. Esmon. 2000. Characterization and Regulation of the 5′-Flanking Region of the Murine Endothelial Protein C Receptor Gene. J. Biol. Chem. 275:12481-12488.

Gu, J.M., Y. Katsuura, G.L. Ferrell, P. Grammas, and C.T. Esmon. 2000. Endotoxin and thrombin elevate rodent endothelial cell protein C receptor mRNA levels and increase receptor shedding in vivo. Blood 95:1687-1693.

Kalaria, V.G., W. Zareba, A.J. Moss, G. Pancio, V.J. Marder, J.H. Morrissey, H.J. Weiss, C.E. Sparks, H. Greenberg, E. Dwyer, R. Goldstein, and L.F. Watelet. 2000. Gender-related differences in thrombogenic factors predicting recurrent cardiac events in patients after acute myocardial infarction. Am. J. Cardiol. 85:1401-1408.

Kinasewitz, G.T., A.C. Chang, G.T. Peer, L.B. Hinshaw, and F.B. Taylor, Jr. 2000. Peritonitis in the baboon: a primate model which stimulates human sepsis . Shock 13:100-109.

Kunzelmann-Marche, C., N. Satta, F. Toti, Y .Zhang, P.P. Nawroth, J.H. Morrissey, and J.M. Freyssinet. 2000. The influence exerted by a restricted phospholipid microenvironment on the expression of tissue factor activity at the cell plasma membrane surface . Thromb. Haemost. 83:282-289.

* Kurien, B.T., J. Newland, C. Paczkowski, K.L. Moore, and R.H. Scofield. 2000. Association of neutropenia in systemic lupus erythematosus (SLE) with anti-Ro and binding of an immunologically cross-reactive neutrophil membrane antigen . Clin.Exp.Immunol. 120:209-217.

* Kurosawa,S., C.T.Esmon, and D.J.Stearns-Kurosawa. 2000. The soluble endothelial protein C receptor binds to activated neutrophils: involvement of proteinase-3 and CD11b/CD18 . J.Immunol. 165:4697-4703.

Leppanen,A., S.P.White, J.Helin, R.P.McEver, and R.D.Cummings. 2000. Binding of glycosulfopeptides to P-selectin requires stereospecific contributions of individual tyrosine sulfate and sugar residues . J.Biol.Chem. 275:39569-39578.

Liang, Z., T. Mather, and G. Li. 2000. GTPase mechanism and function: new insights from systematic mutational analysis of the phosphate-binding loop residue Ala30 of Rab5. Biochem. J. 346:501-508.

Liaw, P.C., P.F. Neuenschwander, M.D. Smirnov, and C.T. Esmon. 2000. Mechanisms by which soluble endothelial cell protein c receptor modulates protein c and activated protein c function. J. Biol. Chem. 275:5447-5452.

Miller, G.J., D.J. Howarth, J.C. Attfield, C.J. Cooke, M.N. Nanjee, W.L. Olszewski, J.H. Morrissey, and N.E. Miller. 2000. Haemostatic factors in human peripheral afferent lymph . Thromb. Haemost. 83:427-432.

Minnema, M.C., A.C. Chang, P.M. Jansen, Y.T. Lubbers, B.M. Pratt, B.G. Whittaker, F.B. Taylor, C.E. Hack, and B. Friedman. 2000. Recombinant human antithrombin III improves survival and attenuates inflammatory responses in baboons lethally challenged with escherichia coli . Blood 95:1117-1123.

* Perera,L., C.Foley, T.A.Darden, D.Stafford, T.Mather, C.T.Esmon, and L.G.Pedersen. 2000. Modeling zymogen protein C . Biophys.J. 79:2925-2943.

Rezaie, A.R. and X. He. 2000. Sodium binding site of factor xa: role of sodium in the prothrombinase complex . Biochemistry 39:1817-1825.

Sanders,T.A., T.de Grassi, G.J.Miller, and J.H.Morrissey. 2000. Influence of fatty acid chain length and cis/trans isomerization on postprandial lipemia and factor VII in healthy subjects (postprandial lipids and factor VII). Atherosclerosis 149:413-420.

Taylor, F.B., Jr. 2000. Diminished oxygen extraction and hypoxia in the presence of normal delivery of oxygen by the microvasculature [editorial] . Crit. Care Med. 28:2163-2164.

Taylor,F., Jr., P.A.Haddad, G.Kinasewitz, A.Chang, G.Peer, and R.C.Allen. 2000. Luminescence studies of the phagocyte response to endotoxin infusion into normal human subjects: multiple discriminant analysis of luminescence response and correlation with phagocyte morphologic changes and release of elastase . J.Endotoxin.Res. 6:3-15.

* Taylor, F.B., D.J. Stearns-Kurosawa, S. Kurosawa, G. Ferrell, A.C. Chang, Z. Laszik, S. Kosanke, G. Peer, and C.T. Esmon. 2000. The endothelial cell protein C receptor aids in host defense against Escherichia coli sepsis. Blood 95:1680-1686.

Taylor,F.B., Jr., H.Wada, and G.Kinasewitz. 2000. Description of compensated and uncompensated disseminated intravascular coagulation (DIC) responses (non-overt and overt DIC) in baboon models of intravenous and intraperitoneal Escherichia coli sepsis and in the human model of endotoxemia: toward a better definition of DIC . Crit Care Med. 28:S12-S19.

Xu, J., D. Qu, N.L. Esmon, and C.T. Esmon. 2000. Metalloproteolytic Release of Endothelial Cell Protein C Receptor. J. Biol. Chem. 275:6038-6044.

Zito,F., F.Drummond, S.R.Bujac, M.P.Esnouf, J.H.Morrissey, S.E.Humphries, and G.J.Miller. 2000. Epidemiological and genetic associations of activated factor XII concentration with factor VII activity, fibrinopeptide A concentration, and risk of coronary heart disease in Men . Circulation 102:2058-2062.

Crystallography Research Program

Guddat,L.W., L.Shan, C.Broomell, P.A.Ramsland, Z.Fan, J.M.Anchin, D.S.Linthicum, and A.B.Edmundson. 2000. The Three-dimensional Structure of a Complex of a Murine Fab (NC10.14) with a Potent Sweetener (NC174): An Illustration of Structural Diversity in Antigen Recognition by Immunoglobulins. J.Mol.Biol. 302:853-872.

Harris,D.L., E.King, P.A.Ramsland, and A.B.Edmundson. 2000. Binding of nascent collagen by amyloidogenic light chains and amyloid fibrillogenesis in monolayers of human fibrocytes. J.Mol.Recognit.13:198-212.

* Hong,L., G.Koelsch, X.Lin, S.Wu, S.Terzyan, A.K.Ghosh, X.C.Zhang, and J.Tang. 2000. Structure of the protease domain of memapsin 2 (beta-secretase) complexed with inhibitor . Science 290:150-153.

* Hong,L., X.C.Zhang, J.A.Hartsuck, and J.Tang. 2000. Crystal structure of an in vivo HIV-1 protease mutant in complex with saquinavir: insights into the mechanisms of drug resistance . Protein Sci. 9:1898-1904.

Parry,M.A., X.C.Zhang, and I.Bode. 2000. Molecular mechanisms of plasminogen activation: bacterial cofactors provide clues. Trends Biochem.Sci 25:53-59.

Ramsland,P.A., L.Shan, C.R.Moomaw, C.A.Slaughter, Z.Fan, L.W.Guddat, and A.B.Edmundson. 2000. An unusual human IgM antibody with a protruding HCDR3 and high avidity for its peptide ligands. Mol.Immunol. 37:295-310.

* Terzyan,S., C.S.Wang, D.Downs, B.Hunter, and X.C.Zhang. 2000. Crystal structure of the catalytic domain of human bile salt activated lipase . Protein Sci. 9:1783-1790.

* Wang, X., S. Terzyan, J. Tang, J.A. Loy, X. Lin, and X.C. Zhang. 2000. Human plasminogen catalytic domain undergoes an unusual conformational change upon activation . J. Mol. Biol. 295:903-914.

Wirz,M., V.Oganesyan, X.J.Zhang, J.Studer, and M.Rivera. 2000. Modulation of redox potential in electron transfer proteins: Effects of complex formation on the active site microenvironment of cytochrome b(5). FARADAY.DISCUSS. 116:221-234.

Developmental Biology Research Program

Bethke,B.D. and B.Sauer. 2000. Rapid generation of isogenic mammalian cell lines expressing recombinant transgenes by use of Cre recombinase. Methods Mol.Biol. 133:75-84.:75-84.

Grollman,E.F., N.J.Philp, P.McPhie, R.D.Ward, and B.Sauer. 2000. Determination of transport kinetics of chick MCT3 monocarboxylate transporter from retinal pigment epithelium by expression in genetically modified yeast . Biochemistry 39:9351-9357.

 Le, Y. and B. Sauer. 2000. Conditional gene knockout using cre recombinase . Methods Mol. Biol. 136:477-85:477-485.

Soukharev, S., W. Berlin, J.A. Hanover, B. Bethke, and B. Sauer. 2000. Organization of the mouse ASGR1 gene encoding the major subunit of the hepatic asialoglycoprotein receptor . Gene 241:233-240.

Free Radical Biology & Aging Research Program

Feng,Z., J.Hong, Q.Qi, Y.Han, B.Wilson, M.Iadarola, N.Tiao, and G.Bing. 2000. Cloning and expression of MP13 gene from rat hippocampus, a new factor related to guanosine triphosphate regulation. Neurosci.Lett. 296:129-132.

Floyd, R.A. and K. Hensley. 2000. Nitrone inhibition of age-associated oxidative damage . Ann. N.Y. Acad. Sci. 899:222-37:222-237.

Floyd,R.A., K.Hensley, and G.Bing. 2000. Evidence for enhanced neuro-inflammatory processes in neurodegenerative diseases and the action of nitrones as potential therapeutics. J.Neural Transm. 60: Suppl 387-414.

Gabbita, S.P., K.A. Robinson, C.A. Stewart, R.A. Floyd, and K. Hensley. 2000. Redox regulatory mechanisms of cellular signal transduction . Arch. Biochem. Biophys. 376:1-13.

Hensley, K., Y. Kotake, H. Sang, Q.N. Pye, G.L. Wallis, L.M. Kolker, T. Tabatabaie, C.A. Stewart, Y. Konishi, D. Nakae, and R.A. Floyd. 2000. Dietary choline restriction causes complex I dysfunction and increased H(2)O(2) generation in liver mitochondria. Carcinogenesis  21:983-989.

Hensley,K., K.A.Robinson, S.P.Gabbita, S.Salsman, and R.A.Floyd. 2000. Reactive oxygen species, cell signaling, and cell injury . Free Radic.Biol.Med. 28:1456-1462.

Hensley, K., K.S. Williamson, and R.A. Floyd. 2000. Measurement of 3-Nitrotyrosine and 5-Nitro-gamma-Tocopherol by high-performance liquid chromatography with electrochemical detection. Free Radic. Biol. Med. 28:520-528.

Hensley, K., K.A. Robinson, Q.N. Pye, R.A. Floyd, I. Cheng, W.A. Garland, and I. Irwin. 2000. CPI-1189 inhibits interleukin 1beta-induced p38-mitogen-activated protein kinase phosphorylation: an explanation for its neuroprotective properties? . Neurosci. Lett. 281:179-182.

Kim, H., G. Bing, W. Jhoo, K.H. Ko, W. Kim, J. Suh, S.Kim, K. Kato, and J. Hong. 2000. Changes of hippocampal Cu/Zn-superoxide dismutase after kainate treatment in the rat. Brain Res. 853:215-226.

Kim,H., W.Jhoo, E.Shin, and G.Bing. 2000. Selenium deficiency potentiates methamphetamine-induced nigral neuronal loss; comparison with MPTP model. Brain Res. 862:247-252.

Kim,H.C., W.K.Jhoo, G.Bing, E.J.Shin, M.B.Wie, W.K.Kim, and K.H.Ko. 2000. Phenidone prevents kainate-induced neurotoxicity via antioxidant mechanisms. Brain Res. 874:15-23.

Kim,H.C., W.K.Jhoo, K.H.Ko, W.K.Kim, G.Bing, M.S.Kwon, E.J.Shin, J.H.Suh, Y.G.Lee, and D.W.Lee. 2000. Prolonged exposure to cigarette smoke blocks the neurotoxicity induced by kainic acid in rats. Life Sci. 66:317-326.

* Kurosawa,S., C.T.Esmon, and D.J.Stearns-Kurosawa. 2000. The soluble endothelial protein C receptor binds to activated neutrophils: involvement of proteinase-3 and CD11b/CD18 . J.Immunol. 165:4697-4703.

Lu,X., G.Bing, and T.Hagg. 2000. Naloxone prevents microglia-induced degeneration of dopaminergic substantia nigra neurons in adult rats. Neuroscience 97:285-291.

Reinke, L.A., D.R. Moore, H. Sang, E.G. Janzen, and Y. Kotake. 2000. Aromatic hydroxylation in PBN spin trapping by hydroxyl radicals and cytochrome P-450. Free Radic. Biol. Med. 28:345-350.

Salsman,S., R.A.Floyd, and K.Hensley. 2000. Dysregulation of phosphorylation cascades in aging and brain disease: The role of reactive oxygen species. Res.Adv.Neurochem. 1:23-33.

Sueishi,Y., M.Kasahara, and Y.Kotake. 2000. Differential pressure effect on bimodal inclusion complex of B-cyclodextrin with a nitroxide radical probe as studied with electron paramagnetic resonance. Chem.Lett. 7:792-793.

Tabatabaie, T., K.L. Graham, A.M. Vasquez, R.A. Floyd, and Y. Kotake. 2000. Inhibition of the cytokine-mediated inducible nitric oxide synthase expression in rat insulinoma cells by phenyl N-tert-butylnitrone . Nitric Oxide 4:157-167.

Tabatabaie, T., A.M. Waldon, J.M. Jacob, R.A. Floyd, and Y. Kotake. 2000. COX-2 inhibition prevents insulin-dependent diabetes in low-dose streptozotocin-treated mice. Biochem. Biophys. Res. Commun. 273:699-704.

* Taylor, F.B., D.J. Stearns-Kurosawa, S. Kurosawa, G. Ferrell, A.C. Chang, Z. Laszik, S. Kosanke, G. Peer, and C.T. Esmon. 2000. The endothelial cell protein C receptor aids in host defense against Escherichia coli sepsis. Blood 95:1680-1686.

Yoon,S.J., Y.H.Koh, R.A.Floyd, and J.W.Park. 2000. Copper, zinc superoxide dismutase enhances DNA damage and mutagenicity induced by cysteine/iron. Mutat.Res. 448:97-104.

Immunobiology and Cancer Research Program

Brugnera,E., A.Bhandoola, R.Cibotti, Q.Yu, T.I.Guinter, Y.Yamashita, S.O.Sharrow, and A.Singer. 2000. Coreceptor reversal in the thymus: signaled CD4+8+ thymocytes initially terminate CD8 transcription even when differentiating into CD8+ T cells. Immunity. 13:59-71.

Hayashi, S., K. Miyake, and P.W. Kincade. 2000. The CD9 molecule on stromal cells . Leuk. Lymphoma 38:265-270.

Hofmeister,J.K., D.Cooney, and K.M.Coggeshall. 2000. Clustered CD20 induced apoptosis: src-family kinase, the proximal regulator of tyrosine phosphorylation, calcium influx, and caspase 3-dependent apoptosis. Blood Cells Mol.Dis. 26:133-143.

Kincade,P.W. 2000. A complete solution? . Immunol.Rev. 175:5-8.

Kincade,P.W., K.L.Medina, K.J.Payne, M.I.Rossi, K.S.Tudor, Y.Yamashita, and T.Kouro. 2000. Early B-lymphocyte precursors and their regulation by sex steroids . Immunol.Rev. 175:128-37.

Kincade,P.W., K.J.Payne, K.S.Tudor, Y.Yamashita, K.L.Medina, M.I.Rossi, and T.Kouro. 2000. Re-evaluation of B lymphocyte lineage differentiation schemes . Curr.Top.Microbiol.Immunol. 251:67-72.

Malone, C.S., L. Patrone, K.L. Buchanan, C.F. Webb, and R. Wall. 2000. An Upstream Oct-1- and Oct-2-Binding Silencer Governs B29 (Igbeta) Gene Expression. J. Immunol. 164:2550-2556.

Medina, K.L., A. Strasser, and P.W. Kincade. 2000. Estrogen influences the differentiation, proliferation, and survival of early B-lineage precursors . Blood 95:2059-2067.

Nadon, N.L. 2000. Aberrant dendrite growth in central nervous system white matter induced by a mutant proteolipid protein transgene. J. Comp. Neurol. 416:262-268.

Nadon,N.L., C.J.Doersen, D.A.Lade, K.Medina, L.Thompson, M.Rohrer, and J.M.Gimble. 2000. Multiple cellular phenotypes in an insertional mutation in mouse affecting bone development. Calcif.Tissue Int. 67:449-454.

Oritani, K., K. Aoyama, Y. Tomiyama, P.W. Kincade, and Y. Matsuzawa. 2000. Stromal cell CD9 and the differentiation of hematopoietic stem/progenitor cells . Leuk. Lymphoma 38:147-152.

Oritani, K., K.L. Medina, Y. Tomiyama, J. Ishikawa, Y. Okajima, M. Ogawa, T. Yokota, K. Aoyama, I. Takahashi, P.W. Kincade, and Y. Matsuzawa. 2000. Limitin: an interferon-like cytokine that preferentially influences B-lymphocyte precursors . Nat. Med. 6:659-666.

Phee, H., A. Jacob, and K.M. Coggeshall. 2000. Enzymatic activity of the Src homology 2 domain-containing inositol phosphatase is regulated by a plasma membrane location. J. Biol. Chem. 275:19090-7.

Sanchez,T.A., I.Habib, B.J.Leland, S.M.Evetts, and J.P.Metcalf. 2000. Zinc finger and carboxyl regions of adenovirus E1A 13S CR3 are important for transactivation of the cytomegalovirus major immediate early promoter by adenovirus. Am.J Respir.Cell Mol.Biol 23:670-677.

Thompson,L.F., C.J.Van De Wiele, A.B.Laurent, S.W.Hooker, J.G.Vaughn, H.Jiang, K.Khare, R.E.Kellems, M.R.Blackburn, M.S.Hershfield, and R.Resta. 2000. Metabolites from apoptotic thymocytes inhibit thymopoiesis in adenosine deaminase-deficient fetal thymic organ cultures . J.Clin.Invest 106:1149-1157.

Tridandapani,S., T.W.Lyden, J.L.Smith, J.E.Carter, K.M.Coggeshall, and C.L.Anderson. 2000. The adapter protein LAT enhances fcgamma receptor-mediated signal transduction in myeloid cells. J Biol Chem. 275:20480-20487.

Tudor, K.S., K.J. Payne, Y. Yamashita, and P.W. Kincade. 2000. Functional assessment of precursors from murine bone marrow suggests a sequence of early B lineage differentiation events . Immunity 12:335-345.

Webb,C.F., Y.Yamashita, N.Ayers, S.Evetts, Y.Paulin, M.E.Conley, and E.A.Smith. 2000. The transcription factor bright associates with Bruton’s tyrosine kinase, the defective protein in immunodeficiency disease . J.Immunol. 165:6956-6965.

Lipoprotein Laboratory

Alaupovic,P. 2000. Management of dyslipidemia after coronary artery bypass grafting . Curr.Opin.Lipidol. 11:369-375.

Johansson,A.C., O.Samuelsson, P.O.Attman, B.Haraldsson, J.Moberly, C.Knight-Gibson, and P.Alaupovic. 2000. Dyslipidemia in peritoneal dialysis–relation to dialytic variables. Perit.Dial.Int. 20:306-314.

Sacks,F.M., P.Alaupovic, L.A.Moye, T.G.Cole, B.Sussex, M.J.Stampfer, M.A.Pfeffer, and E.Braunwald. 2000. VLDL, apolipoproteins B, CIII, and E, and risk of recurrent coronary events in the cholesterol and recurrent events (CARE) trial . Circulation 102:1886-1892.

Molecular and Cell Biology Research Program

Conaway, J.W. and R.C. Conaway. 2000. Structural biology. Light at the end of the channel [comment]. Science 288:632-633.

Conaway,J.W., A.Shilatifard, A.Dvir, and R.C.Conaway. 2000. Control of elongation by RNA polymerase II. Trends Biochem.Sci. 25:375-380.

Dresser,M.E. 2000. Meiotic chromosome behavior in Saccharomyces cerevisiae and (mostly) mammals. Mutat.Res. 451:107-127.

Friedman, L., J.J. Higgin, G. Moulder, R. Barstead, R.T. Raines, and J. Kimble. 2000. Prolyl 4-hydroxylase is required for viability and morphogenesis in Caenorhabditis elegans. Proc. Natl. Acad. Sci.U.S.A 97:4736-4741.

Kamura,T., S.Sato, K.Iwai, M.Czyzyk-Krzeska, R.C.Conaway, and J.W.Conaway. 2000. Activation of HIF1alpha ubiquitination by a reconstituted von hippel-lindau (VHL) tumor suppressor complex . Proc.Natl.Acad.Sci.U.S.A 97:10430-10435.

Kelleher,J.F., M.A.Mandell, G.Moulder, K.L.Hill, S.W.L’Hernault, R.Barstead, and M.A.Titus. 2000. Myosin VI is required for asymmetric segregation of cellular components during C. elegans spermatogenesis . Curr.Biol. 10:1489-1496.

Kohn,R.E., J.S.Duerr, J.R.McManus, A.Duke, T.L.Rakow, H.Maruyama, G.Moulder, I.N.Maruyama, R.J.Barstead, and J.B.Rand. 2000. Expression of multiple UNC-13 proteins in the caenorhabditis elegans nervous system . Mol.Biol.Cell 11:3441-3452.

Koth, C.M., M.V. Botuyan, R.J. Moreland, D.B. Jansma, J.W. Conaway, R.C. Conaway, W.J. Chazin, J.D. Friesen, C.H. Arrowsmith, and A.M. Edwards. 2000. Elongin from saccharomyces cerevisiae . J. Biol. Chem. 275:11174-11180.

Miller,K.G., M.D.Emerson, J.R.McManus, and J.B.Rand. 2000. RIC-8 (Synembryn): a novel conserved protein that is required for G(q)alpha signaling in the C. elegans nervous system. Neuron 27:289-299.

Miller,K.G. and J.B.Rand. 2000. A Role for RIC-8 (Synembryn) and GOA-1 (G(o)alpha) in Regulating a Subset of Centrosome Movements During Early Embryogenesis in Caenorhabditis elegans. Genetics 156:1649-1660.

Moreland, R.J., M.E. Dresser, J.S. Rodgers, B.A. Roe, J.W. Conaway, R.C. Conaway, and J.S. Hanas. 2000. Identification of a transcription factor IIIA-interacting protein. Nucleic Acids Res. 28:1986-1993.

Rand,J.B., J.S.Duerr, and D.L.Frisby. 2000. Neurogenetics of vesicular transporters in C. elegans. FASEB J. 14:2414-2422.

Trelles-Sticken,E., M.E.Dresser, and H.Scherthan. 2000. Meiotic telomere protein ndj1p is required for meiosis-specific telomere distribution, bouquet formation and efficient homologue pairing . J.Cell Biol. 151:95-106.

Wixon, J., M. Blaxter, I. Hope, R. Barstead, and S. Kim. 2000. Caenorhabditis elegans. Yeast 17:37-42.

Molecular Immunology Research Program

Carayannopoulos, M.O., K.N. Potter, Y. Li, J.B. Natvig, and J.D. Capra. 2000. Evidence that human immunoglobulin M rheumatoid factors can Be derived from the natural autoantibody pool and undergo an antigen driven immune response in which somatically mutated rheumatoid factors have lower affinities for immunoglobulin G Fc than their germline counterparts . Scand. J. Immunol. 51:327-336.

Frazer,J.K., D.G.Jackson, J.P.Gaillard, M.Lutter, Y.J.Liu, J.Banchereau, J.D.Capra, and V.Pascua. 2000. Identification of centerin: a novel human germinal center B cell-restricted serpin . Eur.J.Immunol. 30:3039-3048.

Srinivas,P. and M.N.Vijayakumar. 2000. Genetic and transcriptional analysis of a regulatory region in streptococcal conjugative transposon Tn5252. Plasmid 44:262-274.

Tuaillon, N. and J.D. Capra. 2000. Evidence that terminal deoxynucleotidyltransferase expression plays a role in Ig heavy chain gene segment utilization . J. Immunol. 164:6387-6397.

Tuaillon,N. and J.D.Capra. 2000. VHD rearrangements in human immunoglobulin heavy chain minilocus transgenic mice . Eur.J.Immunol. 30:2998-3005.

Wilson, P.C., K. Wilson, Y.J. Liu, J. Banchereau, V. Pascual, and J.D. Capra. 2000. Receptor revision of immunoglobulin heavy chain variable region genes in normal human B lymphocytes . J. Exp. Med. 191:1881-1894.

Protein Studies Research Program

Ermolieff,J., J.A.Loy, G.Koelsch, and J.Tang. 2000. Proteolytic activation of recombinant pro-memapsin 2 (Pro-beta-secretase) studied with new fluorogenic substrates . Biochemistry 39:12450-12456. (Correction to Figure 2 on p.12452 published in v.39 p.16263.)

Ghosh, A.K., D. Shin, D. Downs, G.  Koelsch, X. Lin, J.  J. Ermolieff, and J. Tang. 2000. Design of potent inhibitors for human brain Memapsin 2 (Beta-Secretase). J. Am. Chem. Soc.122:3522-3523.

* Hong,L., G.Koelsch, X.Lin, S.Wu, S.Terzyan, A.K.Ghosh, X.C.Zhang, and J.Tang. 2000. Structure of the protease domain of memapsin 2 (beta-secretase) complexed with inhibitor . Science 290:150-153.

* Hong,L., X.C.Zhang, J.A.Hartsuck, and J.Tang. 2000. Crystal structure of an in vivo HIV-1 protease mutant in complex with saquinavir: insights into the mechanisms of drug resistance . Protein Sci. 9:1898-1904.

Koelsch,G., J.Tang, J.A.Loy, M.Monod, K.Jackson, S.I.Foundling, and X.Lin. 2000. Enzymic characteristics of secreted aspartic proteases of candida albicans. Biochim.Biophys.Acta 1480:117-131.

Lin, X., G. Koelsch, S. Wu, D. Downs, A. Dashti, and J. Tang. 2000. Human aspartic protease memapsin 2 cleaves the Beta-secretase site of Beta-amyloid precursor protein. Proc. Natl. Acad. Sci. USA 97:1456-1460.

* Terzyan,S., C.S.Wang, D.Downs, B.Hunter, and X.C.Zhang. 2000. Crystal structure of the catalytic domain of human bile salt activated lipase . Protein Sci. 9:1783-1790.

* Wang, X., S. Terzyan, J. Tang, J.A. Loy, X .Lin, and X.C .Zhang. 2000. Human plasminogen catalytic domain undergoes an unusual conformational change upon activation . J. Mol. Biol. 295:903-914.

* Indicates publications by more than one department.