Platelets function primarily in hemostasis and thrombosis. Recent studies have expanded the platelet function to innate immune response and tissue homeostasis. My research is focused on the role of platelet receptors in thrombosis, microangiopathy, and the host response to infections.
CLEC-2 is a membrane receptor mainly expressed on platelets. The only known ligand for CLEC-2 is podoplanin, which is expressed on many cell types including alveolar epithelial cells, lymphatic endothelial cells, and podocytes. In healthy animals, podoplanin is not expressed on blood cells and the blood vessel wall. Engagement of CLEC-2 by podoplanin plays an essential role in separation of lymphatic vessels from veins, maintenance of blood vessel integrity, and tumor metastasis.
I have found that CLEC-2 interacts with the platelet receptor GPIb. I have also found that circulating monocytes in septic mice express podoplanin. Deletion of CLEC-2 impairs GPIb-dependent platelet activation and enhances liver dysfunction in sepsis. Thus, I am using various mouse genetic models, constructed cell lines, and recombinant proteins to study the CLEC-2-mediated or -regulated intracellular signaling in the pathogenesis of arterial thrombosis, thrombotic small blood vessel damage, and sepsis. The ultimate goal of my current research is to develop therapeutic approaches to prevent arterial thrombotic occlusion and to protect the liver function in sepsis.
B.S., Suzhou Medical College, Suzhou, Jiangsu, China, 1997
M.S., Soochow University, Suzhou, Jiangsu, China, 2003
Ph.D., University of Oklahoma Health Sciences Center, Oklahoma City, OK, 2011
Shi H, Shao B, Gao L, Venkatesan T, McDaniel JM, Zhou M, McGee S, Yu P, Ahamed J, Journeycake J, George JN, Xia L. Endothelial VWF is critical for the pathogenesis of vaso-occlusive episode in a mouse model of sickle cell disease. Proc Natl Acad Sci U S A 119:e2207592119, 2022 August, PMID: 35969769, PMCID: PMC9407592
Shao B, Hoover CM, Shi H, Kondo Y, Lee RH, Chen J, Shan X, Song J, McDaniel M, Zhou M, McGee S, Vanhoorelbeke K, Bergmeier W, López JA, George JN, Xia L. Deletion of platelet CLEC-2 decreases GPIba-mediated integrin aIIbb3 activation and decreases thrombosis in TTP. Blood, 2022 February, PMID: 35157766, PMCID: PMC9029097
Kondo C, Larabee J, Gao L, Shi H, Shao B, Hoover C, McDaniel M, Ho Y, Silasi-Mansat R, Archer- Harmann S, Azadi P, Srinivasan S, Rezaie A, Borczuk A, Laurence J, Lupu F, Ahamed J, McEver RP, Papin J, Yu Z, Xia L. L-SIGN is a receptor on liver sinusoidal endothelial cells for SARS-CoV-2 virus. 2021.JCI Insight.6(14):148999. PMID: 34291736
Hoover C, Kondo Y, Shao B, McDaniel M, Lee R, Whiteheart SW, Bergmeier W, McEver RP, Xia L. Heightened activation of embryonic megakaryocytes causes aneurysms in the developing brain of mice lacking podoplanin. 2021. Blood.137(20):2756-2769. PMID: 33619517, PMCID: PMC8138551.
Xie Z*, Shao B* (equal contribution), Song J, Hoover C, McDaniel M, Jiang M, Ma Z, Bai X, Ruan C, Xia L. Monocyte upregulation of podoplanin during early sepsis induces complement inhibitor release to protect liver function. 2020. JCI Insight. 5(13): e134749. PMID:32641582, PMCID: PMC7406268.
Shao B, Yago T, Panicker S, Zhang N, Liu Z, McEver R.P. Th1 cells rolling on selectins trigger DAP12-dependent signals that activate integrin aLb2. 2020. J Immunol. 204(1): 37-48. PMID: 31757864, PMCID: PMC6920551.
Panicker S, Yago T, Shao B, McEver RP. Neutrophils lacking ERM proteins polarize and crawl directionally but have decreased adhesion strength. 2020. Blood Adv. 4(14): 3559-3571. PMID: 32761234, PMCID: PMC7422121.
Bergstrom K, Shan X, Casero D, Batushansky A, Lagishetty V, Jacobs JP, Hoover C, Kondo Y, Shao B, Gao L, Zandberg W, Noyovitz B, McDaniel M, Gibson DL, Pakpour S, Kazemian N, McGee S, Houchen CW, Rao CV, Griffin TM, Sonnenburg JL, McEver RP, Braun J, Xia J. Proximal colon-derived O-glycosylated mucus encapsulates and modulates the microbiota. 2020. Science. 370(6515):467-472. PMID: 33093110, PMCID: PMC8132455.
Cardiovascular Biology Research Program, MS 45
Oklahoma Medical Research Foundation
825 N.E. 13th Street
Oklahoma City, OK 73104
Phone: (405) 271-3979
Fax: (405) 271-3137