The Sequencing Facility provides the following primers:
T7, T7 terminator, T3, SP6, BGH Reverse, M13 Forward (-20), M13 Reverse (-27).
Primer sequences can be found here:
M13 Forward GTA AAA CGA CGG CCA GTG
M13 Reverse GGA AAC AGC TAT GAC CAT G
T7 Promoter GTA ATA CGA CTC ACT ATA GGG
T3 Promoter CAA TTA ACC CTC ACT AAA GG
SP6 Promoter TAC GAT TTA GGT GAC ACT ATA G
BGH Reverse TAG AAG GCA CAG TCG AGG
T7 Terminator GCT AGT TAT TGC TCA GCG G
The Sequencing Facility welcomes custom primers that fit our guidelines.
- have a Tm of 55o-60o C
- length of approximately 18-24 bases
- contain no mismatches
- no secondary structure (particularly at the 3′ end)
- no long strings (<4) of any single base, especially G or C