DNA Sequencing

The Sequencing Facility provides the following primers:

T7, T7 terminator, T3, SP6, BGH Reverse, M13 Forward (-20), M13 Reverse (-27).

Primer sequences can be found here:

M13 Forward GTA AAA CGA CGG CCA GTG

M13 Reverse GGA AAC AGC TAT GAC CAT G

T7 Promoter GTA ATA CGA CTC ACT ATA GGG

T3 Promoter CAA TTA ACC CTC ACT AAA GG

SP6 Promoter TAC GAT TTA GGT GAC ACT ATA G

BGH Reverse TAG AAG GCA CAG TCG AGG

T7 Terminator GCT AGT TAT TGC TCA GCG G

The Sequencing Facility welcomes custom primers that fit our guidelines.

• have a T_m of 55^o-60^o C
• length of approximately 18-24 bases
• contain no mismatches
• no secondary structure (particularly at the 3′ end)
• no long strings (<4) of any single base, especially G or C