BIACORE
The BIACORE is an optical biosensor that uses surface plasmon resonance (SPR) to monitor macromolecular interactions. The OMRF BIACORE Core Facility is now equipped with a Biacore T200, which is a versatile platform for comprehensive characterization of molecular interactions in terms of kinetics, affinity, specificity, concentration, immunogenicity, and thermodynamics.
The core facility provides training in the use of the instrument, and assistance with experimental planning and data analysis when needed.
Applications
The Biacore T200 uses surface plasmon resonance (SPR) to enable real-time monitoring of binding interactions in a label-free environment.
Applications include:
- Kinetics/affinity characterization
- Kinetics/affinity screening
- Single-cycle kinetics
- Low-molecular-weight (LMW) interaction analysis
- Fragment screening
- Epitope mapping (with limitations)
- Immunogenicity
- Concentration analysis
- Calibration-free concentration analysis
- Thermodynamics
- Sample recovery MS
Fees
Instrument usage fees
Academic users $300/day
Non-OMRF academic $400/day
Non-academic users $600/day
Training fees
Academic users $300
Non-OMRF academic $400
Non-academic users $500
Consultation
Academic users $75/hour or $300/day (intermittent help)
Non-OMRF academic $85/hour or $350/day (intermittent help)
Non-academic users $125/hour or $500/day (intermittent help)
Facility Fees
Academic users $65/month
Non-OMRF academic $75/month
Non-academic users $100/month
Consumables and other supplies available at cost
Padmaja Mehta-D’souza, Ph.D.
