DNA Sequencing

The OMRF DNA Sequencing facility provides low cost DNA sequencing to OMRF and OUHSC investigators, as well as investigators from other Oklahoma universities and schools. The Facility’s ABI 3730 capillary sequencer is capable of running 180 samples per day using purified plasmid or PCR amplified DNA. Operating hours are Monday through Friday, 8:30 am to 5:00 pm. Samples received before 1:00 are generally ready the next afternoon. The cost is $7 per primer/template pair for OMRF, $8 for OUHSC, and in-state university researchers. The cost is $15 or out of state institutions and for-profit organizations.

For more information, contact Sheryl Christofferson, Room W104, at sherylc@omrf.org or (405) 271-1362, ext 34565.

The Sequencing Facility provides the following primers:

T7, T7 terminator, T3, SP6, BGH Reverse, M13 Forward (-20), M13 Reverse (-27).

Primer sequences can be found here:

M13 Forward GTA AAA CGA CGG CCA GTG

M13 Reverse GGA AAC AGC TAT GAC CAT G

T7 Promoter GTA ATA CGA CTC ACT ATA GGG

T3 Promoter CAA TTA ACC CTC ACT AAA GG

SP6 Promoter TAC GAT TTA GGT GAC ACT ATA G

BGH Reverse TAG AAG GCA CAG TCG AGG

T7 Terminator GCT AGT TAT TGC TCA GCG G

The Sequencing Facility welcomes custom primers that fit our guidelines.

have a T_m of 55^o-60^o C
length of approximately 18-24 bases
contain no mismatches
no secondary structure (particularly at the 3′ end)
no long strings (<4) of any single base, especially G or C

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